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Wednesday, 7 March 2012

Fwd: Abcam - Tips for fixed cell imaging, detection of phosphoproteins and new JAK/STAT pathway card



Tips for fixed cell imaging, detection of phosphoproteins and new JAK/STAT pathway card

Abcam: discover more

Abcam is committed to stay up-to-date with the latest research and trends. We have over 86,000 research products including primary and secondary antibodies, biochemicals, proteins, peptides, lysates and kits.

Five tips for fixed cell imaging

  • Choose the best fixation method for your protein/cellular compartment of interest
  • Select your blocking agent/normal serum based on your protein of interest/species the secondary was raised in
  • Choose a primary antibody recommended for IF/ICC and prepare samples with different antibody dilutions
  • Prepare a secondary antibody-only control and also design other controls
  • Select pre-adsorbed secondary antibodies as they reduce potential cross-reactivity


Donkey polyclonal secondary antibody to rabbit IgG - H&L (FITC) pre-adsorbed (ab98502)
view the full range of PhosphoTracer kit
PhosphoTracer ELISA kits

Abcam's new phosphoELISA range enables you to measure protein phosphorylation more quantitatively than Western blotting ever could.

The protocol is unlike any other as both the analyte and the assay reagents are added to the microplate simultaneously.

In slightly more than an hour, you can measure:

  • AKT 1/2/3 (pS473) with ab119614
  • JNK (pT183/Y185) and JNK (Total) with ab119663
  • STAT1 (pY701) and STAT3 (pY705) and STAT5 (pY694) with ab119678

JAK/STAT Signalwegkarte
Brand new JAK/STAT pathway card

Our latest addition to our extensive poster library shows all key steps in the JAK/STAT signaling pathway.
This pathway is vital to the integrity of organisms and contributes to various cellular processes such as growth, immune response, proliferation and hematopoiesis.

Related links

Fluorescent ligands - for FACS and confocal microscopy | Abcam cell imaging products |
Optiblot - our brand new Western blot range |

Discover more at abcam.com

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